H4受体介导组胺刺激AtT
【关键词】 组胺
【Abstract】 AIM: To clarify the subtype of histamine (HA) receptor which mediates Adrenocorticotropic Hormone(ACTH) release from mouse anterior pituitary tumor cell line AtT20. METHODS: ELISA assay was used to determine the effects of a variety of histamine receptor subtypespecific agonists and antagonists on ACTH release from AtT20 cells. The expression of H4 receptor mRNA on AtT20 cells was detected by RTPCR and the PCR product was sequenced. RESULTS: ① HA (108 mol/L) significantly accelerated the release of ACTH (pmol/L) from AtT20 cells （5.7±1.1 vs 2.7±0.6 in control, P
